The one season trial for 2016/17 will include 64 teams made up of EFL League One and Two clubs, plus an additional 16 category 1 Premier League academy/under-21 sides.
Central to the competition will be the introduction of a new group stage format with 16 regional groups of 4 teams. The top two teams will progress to the knockout stages of the competition with the final staged at Wembley Stadium in April 2017.
Full details for the 2016/17 EFL Trophy will be finalised within the next 14 days.